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Satoshi T If you close to small thing , the depth of field is very thin. I recommend higher ISO value and more close aperture. if you have enough light, use mini trypod or something to hold your camera for slow shutterspeed. When you have more time, I recommend to try again.

EXIF data
Brand
Canon
Model
Canon EOS 500D
Aperture
ƒ/2.2
Focal length
50mm
Shutter speed
1/15s
ISO
800